protein marker  (Shanghai Acmec Biochemical Technology Co Ltd)

Journal: Materials Today Bio

Article Title: Exercise-primed exosomes in an injectable hydrogel promote myocardial repair via angiogenesis and ferroptosis inhibition

doi: 10.1016/j.mtbio.2026.103035

Figure Lengend Snippet: Characterization and distribution of exercise-derived serum exosomes . A, Schematic diagram illustrating the proposed mode of action and therapeutic strategy. B, TEM image showing the typical cup-shaped morphology of isolated exosomes. Scale bar = 100 nm. C, Size distribution profile of exosomes measured by DLS. D, Confocal microscopy image showing the incorporation of exosomes (green) within the hydrogel matrix. Scale bar = 50 μm. E, Cellular uptake of DiO-labeled exosomes (green) by HUVECs in vitro. Nuclei were stained with DAPI (blue). Scale bar = 25 μm. F, Western blot analysis of Exosome positive marker proteins TSG101, CD81, HSP70 and exosome negative marker protein Histone. G, Flowchart for isolating exosomes from the serum of exercise and sedentary mice. H, In vivo distribution and sustained release of exosomes in cardiac tissue of MI-Exos and MI-Exos@Gel-PEG treated mice. I, Quantitative analysis of exosomal fluorescence intensity in heart tissues (n = 3). (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

Article Snippet: F, Western blot analysis of Exosome positive marker proteins TSG101, CD81, HSP70 and exosome negative marker protein Histone.

Techniques: Derivative Assay, Isolation, Confocal Microscopy, Labeling, In Vitro, Staining, Western Blot, Marker, In Vivo, Fluorescence